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Immunoblotting investigations into additional markers involved in ECSC isk under differentiation conditions (ECSC medium removed of growth factors) showed that the levels of all stemness genes (SOX2, NANOG and ALDH1) decreased over time, while E-cadherin and Vimentin apparently increased after long-term culturing (Supplementary Fig
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The cells also become more resistant to lipolysis, the breakdown of stored fat for energy
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